Biomedical Imaging Research Unit


MetaSystems VSlide slide scanner

Applications

  • Whole section scanning
  • Region of interest scanning including setting up a scan field (region of interest) at low magnification and then scanning the ROI at higher magnifcation/resolution.
  • Tissue microarray scanning and on the fly analysis
  • Brightfield, high resolution brightfield (using the monochrome camera) or fluorescence

Cameras

CoolCube1 (colour & monochrome)

 

Specifications

  • Zeiss Axio Imager Z2 fully motorised microscope
  • Egonomic driver for XY control
  • Automated 80 slide feeder
  • Objectives: Dry (2.5x/0.075 NA, 10x/0.45 NA, 20x/0.9 NA, 40x/0.95 NA) Oil (40x/1.3 NA, 63x/1.4 NA)
  • Automated oiler module
  • Marzhauser Motorised 5 position scanning stage
  • MetaCyte acquisition software and stitching
  • On the fly TMA (tissue microarray) analysis
  • Fluorescence filter sets for DAPI, FITC, TxRed, Cy5 and triple viewing set. Details available in document below.
metasystemsvslide_400px

Examples

If you want to see some examples which illustrate what can be done with this system, please take a look at the movies below which were created by Luke Hammond from QBI Microscopy using his VSlide.

Support files

ImageJ macros for cropping borders from extracted images:

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brightfield
(1.0 kB, TEXT)
*
fluorescence
(994.0 bytes, TEXT)
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Using the ImageJ macros
(276.6 kB, PDF)

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IrfanView - Instructions for cropping extracted images
This document explains how to use open source software IrfanView to crop off the white/black boxes from extracted images. (740.1 kB, PDF)

Funded by

Successful grant application by Professor Mike Dragunow (Dept of Pharmacology & Centre for Brain Research) to Gravida: National Centre for Growth and Development

 

Room extension number

82090

 

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