Biomedical Imaging Research Unit
Light sheet microscopy - PhaseView Alpha3 Facility Edition
The PhaseView Alpha 3 Facility Edition light sheet microscope is fully motorised with a large number of different illlumination and detection objective lenses. It is designed for flexibility and can image a wide range of sample sizes of different refractive indices. The sample must be transparent in order to be able to successfully image it. See the brochure
Applications
- Imaging optically cleared samples, e.g. organoids, spheroids, tissue blocks
- Live imaging, e.g. zebrafish embryos
Specifications
- LASERS: diode (405nm, 488nm, 561nm, 638nm, 730nm, 785nm)
- Camera - Hamamatsu Orca Flash 4 V3 sCMOS
- Filter sets: ET440/40x, ET525/50m, ET600/50m, ET690/50m, ET775/50x, RET792LP
- Focus sweeping module
- Dynamic focus module - for chromatic aberration due to wavelength
- XY Tiling X:25mm Y:25mm range
- Okolab incubation system for environmental control.
- Multiple chambers available for different sample sizes and volumes
- Specialised holders for capillary tubes, organoids, etc.
Illumination objective lenses - for creating light sheet
| Objective lens | Light sheet thickness |
| 2x | 9 microns |
| 5x | 4 microns |
| 10x | 1.5 microns |
| 20x | 0.8 microns |
Detection objective lenses - for imaging
| XLFLUOR 2X/340/ 0.14 NA LOW MAGNIFICATION FL - WD approximately 20mm |
| XLFLUOR 4X/340 / 0.28 NA LONG WD SEMI APOCHROMAT - WD approximately 29mm |
| UMPLFLN 10X/ 0.3 NA W UMPLAN SEMI APOCHROMAT WATER IMMERSION - WD 3.5mm |
| UMPLFLN 20X/ 0.5 NA W UMPLAN SEMI APOCHROMAT WATER IMMERSION - WD 3.5mm |
| XLSLPLN 25X/ 0.95 NA SVMP2 25X SCALEVIEW IMMERSION (RI: 1.33-1.41) - WD 8.0mm |
| LUMPLFLN 40X/ 0.8 NA W LWD UM PLAN SEMI APOCHROMAT - WD 3.3mm |
| XLPLN 10X/ 0.6 NA SVMP (0.6NA/8WD SCALEVIEW (RI:1.33-1.52) - WD 8.0mm |
| XLUMPlanFL N 20x/1.0 NA WATER IMMERSION - WD 2.0mm |
Emission filters
| Emission filter ET440/40x - for DAPI, Hoechst, Alexa Fluor 405 |
| Emission filter ET525/50m - for GFP, Alexa Fluor 488, DyLight 488, FITC |
| Emission filter ET600/50m - for DsRed, mCherry, Alexa Fluor 568, Alexa Fluor 594, Abberior STAR Orange, TRITC |
| Emission filter ET690/50m - for Alexa Fluor 647, Abberior STAR RED, Cy5 |
| Emission filter ET775/50x - for Alexa Fluor 680 |
| Emission filter RET792/LP - for Alexa Fluor 750 |
Software
- QtSPIM - acquisition
- LINDA - acquisition
- Imaris - for 3D visualisation and segmentation. Facility licence including file conversion, stitcher and deconvolution
YouTube video explaining how to convert, stitch and render data sets
YouTube video explaining how to create animations and snapshots
Equipment charges - users from The University of Auckland
- Optically cleared samples - $62.40 per hour
- Live imaging - $41.60 per hour for the first 3 hours, $20.80 per hour thereafter
Contact person
Jacqueline Ross
Lead Technologist, Technical Services
Email: jacqui.ross@auckland.ac.nz
Telephone: 923 7438 or Ext 87438
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