Biomedical Imaging Research Unit

Nanolive 3D Cell Explorer holotomographic microscope

Imaging capabilities

  • 3D holotomographic imaging - label-free
  • Fluorescence imaging - 2D only
  • Quantitative phase analysis - calibration available
  • Colour-coding (“painting”) based on refractive index
  • Live cell imaging - up to 96 hours

Specifications

Nanolive_600px
  • 60x/0.8 NA objective lens, WD 300 microns
  • High resolution – lateral 200nm, axial 400nm
  • Field of view 85 x 85 x 30 microns
  • Okolab incubation system - temperature and carbon dioxide control, compatible with microfluidics and perfusion systems
  • Camera – CMOS, 165 frames/sec at 1024 x 1024 pixels
  • Class I laser (520nm) low power
  • One 3D reconstruction every 2 seconds
  • Cool LED illumination source for fluorescence
  • Triple-band filter set – FITC/TRITC/Cy5

Specimen requirements

•Live or fixed cells grown on coverslips or in ibidi dishes (<30 microns thick)

•Tissue sections 5-10 microns thick unless very transparent

•Mounting media for tissue sections must be aqueous-based, e.g. Fluoromount, Prolong Gold

•Media for live imaging should be low scatter, wide range of media compatible with the system

•Samples cannot be dry

Documentation

Useful supporting materials for the system are available here including "how to" videos. Printed copies are also available in the microscope room.

Contact person