Biomedical Imaging Research Unit

Zeiss Axio Imager M2 fluorescence microscope

Cameras

  • Photometrics Prime sCMOS monochrome
  • Jenoptik Prokyon sCMOS colour

Specifications

The microscope has a motorised focus drive, filter wheel, shutters, condenser and objective turret. It has an LED light source for transmitted light and XCite light source for fluorescence imaging. In addition to the standard condenser, the system has an oil immersion condenser suitable for high resolution darkfield imaging.

Objective lenses

Wide variety of objective lenses from 5x dry to 100x oil immersion including long working distance water lenses. Contrast techiques include phase contrast, darkfield, polarised light and differential interference contrast.

Fluorescence filter sets

  • DAPI bandpass, DAPI longpass
  • FITC bandpass, FITC longpass
  • TRITC bandpass
  • Texas Red bandpass
  • Cy5 bandpass
  • BV480 (similar to CFP) - coming soon!

ZeissAxioImagerWeb

Software and application modules

MetaMorph Premier version 7.10

Application modules - Angiogenesis Tube Formation • Cell Cycle • Cell Health • Cell Scoring • Multi Wavelength Cell Scoring • Count Nuclei  • Neurite Outgrowth •  Multi Dimensional Motion Analysis

Documentation from Molecular Devices is available for University of Auckland staff/students here. Please note that this is a secure page and these files should not be shared without permission from a BIRU staff member.

 

Gryphax

  • For use with the Jenoptik Prokyon colour camera

Equipment charge - for users from The University of Auckland

  • Microscope and software use - $10.50 per hour
  • Software only - free of charge (note that priority is given to microscope use)

Contact person

Jacqueline Ross

Lead Technologist / Technical Services

Email: jacqui.ross@auckland.ac.nz