Biomedical Imaging Research Unit

MetaXpress documentation

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MetaXpress basics
Explains how to use the MetaXpress application modules (1.4 MB, PDF)

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Angiogenesis module
Identifies and measures tubes (long thin objects) and nodes (connecting points between tubes) in a single wavelength (623.9 kB, PDF)

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Cell cycle module
Cell-by-cell cell cycle classification into G0/G1, S phase, G2, early mitosis, late mitosis, and (optional) apoptosis using 1-3 wavelengths (966.7 kB, PDF)

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Cell scoring module
Used to analyze cells imaged in 2 wavelengths. Will score cells as positive (green) or negative (red) for wavelength 2. (1.6 MB, PDF)

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Multi wavelength cell scoring module
Used to analyze cells imaged in 1-7 wavelengths. With 2 or more wavelengths, it will score cells as positive or negative for each wavelength and assign it a “profile” of its score in each wavelength. (992.5 kB, PDF)

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Micronuclei module
Discriminates phenotypes based on Cell Morphology, number of nuclei, Distance of Micronuclei from Nucleus, Micronuclei vs. “Blebs” or “buds” (861.5 kB, PDF)

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Neurite outgrowth module
Identifies and measures processes connected to cell bodies (998.9 kB, PDF)

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Transfluor module
Used to analyze the number and intensity of pits (small) and/or vesicles (larger and brighter) per image and per cell. (2.3 MB, PDF)

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Translocation module
Used to measure intensity movement from one compartment to another (for instance the nucleus to cytoplasm) (1.0 MB, PDF)

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Multi wavelength translocation module
Measures nuclear translocation in assays with a nuclear stain and 1-6 probes of interest. Scores cells as positive or negative for each wavelength based on correlation with the nuclear stain. Assigns profiles to cells based on their score in each wavelength (571.1 kB, PDF)