Biomedical Imaging Research Unit
Nikon TE2000E inverted fluorescence microscope
Camera
- Nikon Digital Sight DS-Ri2 CMOS sensor colour camera
- Photometrics Evolve EMCCD camera (monochrome) - see tutorial
- Basler ace USB3 Camera acA1920-155um, Mono, 1920 x 1200 pixels - for high speed imaging
Applications
- Brightfield microscopy
- Phase contrast microscopy
- Hoffman modulation contrast
- Differential interference contrast
- Fluorescence microscopy
- Time-lapse live cell/tissue imaging
- Fluxion BioFlux 200 available for use with the microscope - enables application of shear stress to cells. For more information...
Software
- NIS Elements AR (Advanced Research) - includes 6D (6 dimensional imaging), EDF (Extended depth of focus) modules
- Free NIS Elements Viewer for Windows or Mac downloadable from here
Specifications
- Motorised microscope stand: focus (z stage), shutters, objective turret and filter wheel + Prior Proscan II motorised XY stage
- Ludl shutters for fluorescence and transmitted light
- Ludl motorised exciter filter wheel - filters for Fura2, CFP/YFP FRET setup, ND filters (80%, 63%, 50%, 25%)
- Filter sets: Semrock zero pixel shift filters: DAPI, FITC, GFP, Texas Red, Fura2, CFP/YFP FRET set-up
- Objectives: brightfield/fluorescence (4x/0.2 NA), phase contrast/fluorescence (10x/0.3 NA, 20x/0.45 NA, 40x/0.6 NA), Hoffmann modulation contrast (20x/0.25 NA, 40x/0.4 NA), brightfield/fluorescence/DIC (40x/1.3 NA oil immersion & 60x/1.25NA oil immersion), 20x/0.75 NA multi-immersion, 60x/1.27 NA water immersion (coverslip adjustment), 60x/0.7 NA ELWD dry
- Solent incubation system (heating and 5% carbon dioxide in air) for live cell/tissue imaging
Equipment charges - users from The University of Auckland
- $11.87 per hour for standard use
- $6.78 per hour for time-lapse imaging after 4 hours of use
Contact person
Jacqueline Ross
Lead Technologist, Technical Services
Email: jacqui.ross@auckland.ac.nz
Telephone: 923 7438 or Ext 87438
Connect with us
-
SCHOOLS, DEPARTMENTS AND CENTRES