Biomedical Imaging Research Unit

Molecular Devices ImageXpress Micro XLS

High content screening system

Fast automated acquisition
Automated customised analysis routines
Hardware (laser-based) autofocus capability
Software (image-based) focusing capability
Multi-well plates up to 1536 wells
Chamber slides
Fluorescence imaging
Brightfield imaging
Phase contrast imaging (20x only)
Live cell imaging - multi-site, multi-wavelength
Z series
Tile-scanning

Specifications

Camera - Andor Zyla CMOS

Objective lenses

2x/0.1 NA CFI Plan Apochromat
4x/0.2 NA S Fluor
10x/0.3 NA Plan Fluor
20x/0.45 NA CFI Super Plan Fluor ELWD ADM (phase contrast)
40x/0.6 NA S Plan Fluor ELWD

Three slide holder - for chamber slides

Environmental control - heating and carbon dioxide gas supply

Light sources

Lumencor Spectra X configurable light engine - fluorescence imaging (filter specifications below)
100 W Halogen lamp - transmitted light

Excitation and emission filters

ImageXpress_FilterSpecifications.pdf
List of excitation and emission filters for Molecular Devices ImageXpress High Content Screening system (69.9 kB, PDF)

Software and application modules

MetaXpress, Custom Module Editor, Autoquant deconvolution

Application modules - Angiogenesis • Cell Cycle • Cell Health • Live/Dead • Multiwavelength Cell Scoring • Neurite Outgrowth • Transfluor • Translocation • Multi Wavelength Translocation • Micronuclei • Granularity

Documentation from Molecular Devices is available for University of Auckland staff/students here. Please note that this is a secure page and these files should not be shared without permission from a BIRU staff member.

Specimen requirements

The IXM microscope stage requires plates to have a base footprint of 128mm x 86mm, with at least 3 square corners present. Please check that your plate meets these requirements.

Please note - the NUNC 142475 24 well plate and the NUNC 150628 12 well plate do not fit the stage so cannot be used as three of the corners are cut off.