Biomedical Imaging Research Unit

Zeiss LSM 710 inverted confocal microscope

Specifications

Lasers: 405nm diode, Argon, diode-pumped solid state (561nm), Helium-Neon (633nm)
Excitation wavelengths: 405, 458, 488, 514, 561, 633nm
Modes: xy, xyz, xz, xt, xyt, lambda
Detectors: 1 spectral, 2 variable band pass, 1 transmitted light
Sequential and online unmixing
Resolution: up to 6144 x 6144 pixel resolution
Objectives: Dry (5x/0.16 NA, 10x/0.45 NA, 20x/0.8 NA), Water (25x/0.8 NA, 40x/1.1 NA), Oil (25x/0.8 NA, 40x/1.3 NA, 63x/1.4 NA), Glycerol (25x/0.8 NA, 63x/1.3 NA), Silicone oil (63x/1.3 NA)
Differential interference contrast optics
Motorised XY stage: tile scanning - StitchArt Plus

Stage-top incubator

The stage-top incubator provides heating ranging from ambient temperature, i.e. 22 degrees Celsius, up to 60 degrees Celsius with carbon dioxide regulation.

The incubation system is controllable via the microscope's TFT interface.

A number of stage inserts are available including Petri dish, 6 well plate, 12 well plate, 48 well plate and 96 well plate.

A holder for chamber slides is also provided.

There are objective heaters for the 40x/1.1 NA water immersion objective and the 63x/1.4 NA oil immersion objective. Objective heaters are not required for dry objective lenses.

Multipoint imaging is possible.