Biomedical Imaging Research Unit

Abberior Facility Line Stimulated Emission Depletion (STED) microscope

STEDsystem_800px

Specifications

Microscope stand - Olympus IX83 inverted microscope

Contrast technique - differential interference contrast (DIC) for all objective lenses.

Fluorescence light source - CoolLED pE-4000; filter sets Quad-Band BrightLine DAPI/FITC/Cy3/Cy5, DAPI/TEXAS RED, FITC/Cy5, Cy7

Objective lenses Confocal - 4x/0.13 NA UPLFLN, 10x/0.3NA UPLFLN, 20x/0.5 NA UPLFLN, 20x/0.85 NA UPLSAPO oil immersion, 40x/1.4 NA UPLXAPO oil immersion, 60x/1.42 NA UPLXAPO oil immersion, 60X/1.3 NA UPLSAPO silicone oil immersion, 60x/1.2 NA UPLSAPO water immersion

Objective lenses STED -  60x/1.42 NA UPLXAPO oil immersion, 60X/1.3 NA UPLSAPO silicone oil immersion, 60x/1.2 NA UPLSAPO water immersion

Lasers - excitation wavelengths 405nm (CW), 485nm (pulsed), 561nm (SHG pulsed), 640nm (pulsed)

STED Laser - 775nm

Detectors - APD, spectral

Advanced features - Easy3D, adaptive illumination (ResCUE, DyMin), adaptive optics, FCS/FLIM

Other options - Olympus motorised XY stage for tile-scanning and multi-position imaging, Okolab stage-top incubation system for live imaging, ZDC autofocus

 

Contact people

Jacqueline Ross

Lead Technologist, Technical Services

Email: jacqui.ross@auckland.ac.nz