Biomedical Imaging Research Unit
The Olympus SLIDEVIEW VS200 is an automated slide-scanner with a range of transmitted light techniques available as well as fluorescence imaging. It has a slide-loader capable of loading up to 180 slides, automatic sample detection (including deep learning) and an integrated barcode reader.
Both transmitted light and fluorescence light sources are LED. For fluorescence, the model is the XYLIS XT720S.
In addition to slide trays for standard slides, there are 2 trays for 2”x3” for 3x slides, 1 tray for 3”x4” for 1x slides, 1 tray for 4”x5” for 1x slides to accommodate the less commonly used sizes.
Objective lenses
- 2x - for brightfield macro overview
- 4x - for fluorescence macro overview
- 10x/0.3 NA UPFLN (also phase contrast)
- 20x/0.8 NA UPLXAPO
- 20x/0.5 NA UPLFLN (also phase contrast)
- 40x/0.95 NA UPLXAPO
Cameras
- Olympus colour camera - integrated 2/3 inch CMOS, 3.45 x 3.45 micron pixel size, 8bit, high sensitivity, high resolution
- Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera USB version, 6.5 micron pixel size
Fluorescence filter sets
| Filter name | Fluorophores | Excitation Filter | Emission Filter |
| DAPI | DAPI, Hoechst, Alexa Fluor 350 | 365/20 | 450/40 |
| CFP | CFP, BV480, Cerulean, Alexa Fluor 430 | 436/20 | 473/24 |
| FITC | FITC, Alexa Fluor 488 | 490/20 | 525/36 |
| YFP | YFP | 500/20 | 540/24 |
| Cy3 | Cy3, Alexa Fluor 546, Alexa Fluor 555 | 555/25 | 590/20 |
| mCherry | mCherry, Alexa Fluor 594, Texas Red | 575/25BP | 645/75 |
| Cy5 | Cy5, Alexa Fluor 647/633, STAR RED/635P | 645/30 | 700/50 |
| Cy7 | Cy7, Alexa Fluor 750 | 725/40 BP | 800/40BP |
Equipment charges - users from The University of Auckland
Subscription rate: $52.00 per hour (10 hours minimum) – pay in advance
Casual rate: $61.73 per hour – pay as you go
Contact person
Ratish Kurian
Senior Technologist/IT Systems Specialist BIRU
Email: r.kurian@auckland.ac.nz
Phone: +64 (0) 9 923 6195
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