Biomedical Imaging Research Unit

Zeiss LSM 800 Airyscan confocal microscope

Specifications

Microscope stand - Axio Observer 7 inverted microscope

Contrast technique - differential interference contrast (DIC) for all objective lenses.

Fluorescence light source - Colibri 7 LED; filter sets 90 HE LED, 91 HE LED, DAPI/Texas Red

Objective lenses - 10x/0.45 NA, Plan Apochromat, 20x/0.8 NA Plan Apochromat, 40x/1.3 NA Plan Apochromat oil immersion, 63x/1.4 NA Plan Apochromat oil immersion

Lasers - excitation wavelengths 405nm, 488nm, 561nm, 640nm

Detectors - 2 x GaAsp-PMT detectors, 1 x Airyscan module, 1 x transmission

Other options - Marzhauser motorised XY stage for tile-scanning and multi-position imaging.

Stage-top incubator

The stage-top incubator provides heating ranging from ambient temperature, i.e. 22 degrees Celsius, up to 60 degrees Celsius with carbon dioxide regulation.

The incubation system is controllable via the microscope's TFT interface.

A number of stage inserts are available including Petri dish, 6 well plate, 12 well plate, 48 well plate and 96 well plate.

A holder for chamber slides is also provided.

There are objective heaters for the 40x/1.1 NA water immersion objective and the 63x/1.4 NA oil immersion objective. Objective heaters are not required for dry objective lenses.

Multipoint imaging is possible.

What stage inserts can I use with the stage-top incubator?

HeatingInserts.pdf
(175.0 kB, PDF)

Software

ZEN 2.6
Download a free lite version of ZEN for viewing and processing your images.
ZEN Knowledge Base - useful video guides to how to use the system, particularly for tile-scanning/stitching capabilities.