Biomedical Imaging Research Unit

Olympus FV4000 confocal microscope

Applications

  • Slide imaging
  • Live imaging
  • Tile-scanning/multipoint imaging

Specifications

  • Fully motorised upright microscope
  • LASERS: diode (405nm,  488nm,  561nm, 640nm, 730nm)
  • Galvanometer and high speed resonant scanner
  • Detectors: Next-Generation SilVIR detectors - 4 spectral including specialised red-shifted detectors and one transmitted light
  • Modes: xy, xyz, xt, xyt, lambda
  • Spectral unmixing
  • Filter sets for viewing specimens
    IX3-FBVWXL (violet excitation; longpass emission)
    IX3-FGFPXL (blue excitation; bandpass emission)
    IX3-FGWXL (green excitation; longpass emission)
  • Objective lenses: dry (10x/0.40 NA, 20x/0.8 NA, 40x/0.95 NA), oil immersion (60x/1.42 NA), water-immersion (20x/1.0 NA)
  • Nomarski differential interference contrast (DIC) available for all objective lenses
  • Marzhauser motorised XY stage, fully controllable through software
  • Newport antivibration table
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Environmental control for live imaging

  • Ludin environmental control system for both heating and cooling
  • Carbon dioxide supply available
  • Full light-tight enclosure

Software

CellSens

Room extension number

82229

Equipment charges - users from The University of Auckland

  • Fixed samples - $67.60 per hour
  • Live imaging - $41.60 per hour for the first 3 hours, $20 per hour thereafter

Contact person

Sandy Chen

Technologist, Technical Services

Email: sandy.chen@auckland.ac.nz

Telephone: 923 6473 or Ext 86473