Biomedical Imaging Research Unit

Olympus FV1000 confocal microscope - live cell imaging


  • Live Cell Imaging
  • Uncaging
  • Photoactivation
  • 3D imaging


    Argon ion multi-line (458nm, 488nm, 514nm)
    Helium Neon: 543nm, 633nm
    Diode-pumped 405nm (for imaging and photoactivation/bleaching)
  • Twin scanner system. SIM scanner for simultaneous bleaching and imaging capability
  • Detectors: 2 spectral (variable bandpass), 1 filter-based, and one transmitted light + 2 high sensitivity detectors (HSD) for green/red and red/far-red
  • Spectral unmixing
  • Modes: xy, xyz, xt, xyt, lambda
  • Resolution: up to 4096 x 4096 pixel resolution
  • Filter blocks for viewing specimens: DAPI, FITC, TRITC, CFP
  • Two separate nosepieces, one for water-immersion long working distance objectives and the other for dry and oil immersion objectives (for imaging slides)
  • Objectives: long working distance water-immersion (10x, 20x, 40x/ 0.8NA, 60x/ 0.9NA), dry (4x, 10x, 20x), silicone oil immersion (60x/ 1.3 NA) and oil immersion (50x, 60x/ 1.35NA, 100x/1/4 NA)
    Specialised 10x/0.6 NA XPLN SVMP lens, working distance 8mm, refractive index (RI) immersion capability from 1.33-1.52
  • Solent incubation system with heating and 5% carbon dioxide supply
  • Kinetic Systems anti-vibration table



  • FluoView 4.2
  • Complete off-line licensed copy available in BIRU computer room for postprocessing.
  • Free FluoView viewer available for users. You can borrow the BIRU CD or download it directly from the Olympus website here.
  • Look here for other software packages available within the BIRU for processing confocal datasets.

Funded by

Bequest to the Faculty of Medical and Health Sciences

Room extension number



Contact people

Jacqueline Ross

Lead Technologist, Technical Services