Biomedical Imaging Research Unit


Olympus FV1000 confocal microscope - live cell imaging

Applications

  • Live Cell Imaging
  • FRAP, FRET
  • Uncaging
  • Photoactivation
  • 3D imaging

Specifications

OlympusLCIS_HSD_whole_microscope_600px
  • LASERS:
    Argon ion multi-line (458nm, 488nm, 514nm)
    Helium Neon: 543nm, 633nm
    Diode-pumped 405nm (for imaging and photoactivation/bleaching)
  • Twin scanner system. SIM scanner for simultaneous bleaching and imaging capability
  • Detectors: 2 spectral (variable bandpass), 1 filter-based, and one transmitted light + 2 high sensitivity detectors (HSD) for green/red and red/far-red
  • Spectral unmixing
  • Modes: xy, xyz, xt, xyt, lambda
  • Resolution: up to 4096 x 4096 pixel resolution
  • Filter blocks for viewing specimens: DAPI, FITC, TRITC, CFP
  • Two separate nosepieces, one for water-immersion long working distance objectives and the other for dry and oil immersion objectives (for imaging slides)
  • Objectives: long working distance water-immersion (10x, 20x, 40x/ 0.8NA, 60x/ 0.9NA), dry (4x, 10x, 20x), silicone oil immersion (60x/ 1.3 NA) and oil immersion (50x, 60x/ 1.35NA, 100x/1/4 NA)
    Specialised 10x/0.6 NA XPLN SVMP lens, working distance 8mm, refractive index (RI) immersion capability from 1.33-1.52
  • Solent incubation system with heating and 5% carbon dioxide supply
  • Kinetic Systems anti-vibration table

 

Software

  • FluoView 4.2
  • Complete off-line licensed copy available in BIRU computer room for postprocessing.
  • Free FluoView viewer available for users. You can borrow the BIRU CD or download it directly from the Olympus website here.
  • Look here for other software packages available within the BIRU for processing confocal datasets.

Funded by

Bequest to the Faculty of Medical and Health Sciences

Room extension number

86728

 

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