Biomedical Imaging Research Unit

Cytek Aurora CS cell sorter

The Cytek Aurora CS Cell Sorter is a spectral sorter that detects the full spectrum of emitted light, which enables a large number of parameters to be assessed simultaneously. This machine is the first in the southern hemisphere to be installed inside a Class II biosafety cabinet, providing a safe aseptic sorting environment for the user and samples.  

Researchers are welcome to utilise this machine to analyse and/or sort samples. Please contact the sorter operator (contact details below) to make a booking. 

Specifications

AuroraCS_cell_sorter

 

Configuration

  • 5 lasers (UV, Violet, Blue, Yellow-Green and Red)
  • 67 detection channels (64 fluorescence channels, FSC, blue laser SSC and violet laser SSC)

 

Lasers

  • 355 nm 20 mW
  • 405 nm 100 mW
  • 488 nm 50 mW
  • 561 nm 50 mW
  • 640 nm 80 mW

 

Detectors

  • APD detector arrays for each laser

 

Nozzles

  • 70, 85, 100, 130um 

 

Collection vessels

  • 1.5ml Eppendorfs and 5ml tubes – up to 6-way sort
  • 15ml Falcon tubes – up to 2-way sort
  • 96-well and 384-well plates – single and multi-cell sort

Software

SpectroFlo® CS ver 1.2.1.1

Equipment charges - users from The University of Auckland

The Aurora CS is charged out at $1.88 per minute (equivalent to $112.80 per hour). There is an additional setup cost of $56.54 per day, which is shared equally between the day’s users (for sorting only). These rates apply only to users from The University of Auckland.

FlowJo and SpectroFlo® CS software are available for use by BIRU flow cytometry facility users without additional charge. A charge of $100.00 per annum applies for software only use.

Contact person

Sandy Chen

Technologist, Technical Services

Email: sandy.chen@auckland.ac.nz

Telephone: 923 6473 or Ext 86473