School of Medical Sciences


Nutrition toxicology

Principal investigator


Co-investigator


Research


Research has focused on the role of metabolism in toxicology and the development in in vitro and in vivo models to assess toxicity. In particular, the balance between protective and detrimental pathways has been studied. Several methods to alter this balance have been investigated: chemical modification, biochemical inhibition and altered expression of the enzymes involved in the bioactivation or detoxification of foreign compounds. Expertise includes the use of cell cultures in vitro and experimental animals, techniques to separate xenobiotics and their metabolites (hplc and tlc), chemical analysis of those metabolites (Mass spectrometry) and methods to determine enzyme activity and expression (Western blotting).

Selected references


Helsby N., Zhu S., Pearson A., Tingle M., Ferguson L. (2000) Studies on the mechanism of cancer protection by wheat bran: ability to modulate xenobiotic metabolising enzymes. Mutation Research in press.

McPerson R., Tingle M., Ferguson. (2001) Effect of chronic dietary exposure to 2-Amino-3-Methyl-Imidazo[4,5-f]Quinoline on xenobiotic metabolising enzymes in the male Fischer 344 rat. The characterisation of a model for chemoprevention studies. Eur. J. Nutr. (submitted).