Biomedical Imaging Research Unit

Becton Dickinson FACS Aria II SORP cell sorter

The system operates aseptically and is compatible with a variety of collection vessels such as multiwell plates and tubes for subsequent tissue culture. Researchers requiring cells to be sorted must arrange a time with the sorter operator (contact details below), who will sort your cells for you.

Specifications

BD_Aria_II_700px

Configuration

  • 4 Laser 15 Colour (4B-3R-3UV-5G)

Lasers

  • Coherent 355nm 20mW
  • Coherent Sapphire 488nm 100mW
  • Coherent Sapphire 552nm 100mW
  • Coherent 640nm 40mW

Detectors

  • Forward scatter - photodiode
  • Side scatter and fluorescence
        - photomultiplier tubes 

Nozzles

  • 50, 70, 85, 100um

Collection vessels

  • 1.5ml Eppendorfs, 5ml tubes, 15ml tubes
  • 6-well to 96-well plates
  • microscope slides

Laser lines and filters

Laser line Detector Mirror Emission Filter Fluorophores
355nm Trigon   450/50 BP Alexa Fluor 350, DAPI, Hoechst Blue, Indo-1 (Violet), Pacific Blue, Live/Dead Fixable Blue, Vibrant DyeCycle Violet
355nm Trigon 505 LP 530/30 BP Indo-1 (Blue), Live/Dead Fixable Aqua, Pacific Orange
355nm Trigon 635 LP 670/50 BP Hoechst Red
         
488nm Octagon 505 LP 530/30 BP Alexa Fluor 488, FITC, GFP, BODIPY, CFSE, CMFDA, FcOxyBurst Green,
Fluo3, Fluo4, Live-Dead Fixable Green, SYBR Green, Sytox-Green,
Vibrant DyeCycle Green, ZsGreen1
488nm Octagon 550 LP 575/25 BP 488-PE, PI, Vibrant DyCycle Orange
488nm Octagon 600 LP 610/20 BP PI
488nm Octagon 655 LP 670/50 BP PE-Cy5, PerCP, PerCP-Cy5-5, 488-PE-Cy5-5, PI
         
552nm Octagon 550 LP 585/15 BP DsRed, PE, Cy3, dTomato, PI, RFP, Sytox-Orange
552nm Octagon 600 LP 610/20 BP PE-Texas Red, PI, Alexa Fluor 568, Live-Dead Fixable Red,
PE-Alexa Fluor 610
552nm Octagon 635 LP 660/20 BP 552-PE-Cy5, 7AAD, Nile Red, PE-Alexa Fluor 647
552nm Octagon 685 LP 710/50 BP PE-Alexa Fluor 680, PE-Cy5-5
552nm Octagon 750 LP 780/60 BP PE-Cy7
         
640nm Trigon   670/30 BP APC, Alexa Fluor 633, Alexa Fluor 647, Live-Dead Fixable Far Red,
Sytox-Red, Topro3
640nm Trigon 685 LP 710/50 BP Alexa Fluor 680, Alexa Fluor 700, AF660, APC-AF680, APC-Cy5-5,
DRAQ5
640nm Trigon 735 LP 780/60 BP APC-Cy7, APC-H7, AF-750APC

Software

BD FACSDiva version 6.1.3 

User charges

The FACSAria II is charged out at $2.13 per minute (equivalent to $140 per hour). There is an additional setup cost of $70 per day, which is shared equally between the day’s users. These rates apply only to users from The University of Auckland.

 

Contact person