School of Medical Sciences


RAGE signalling in steoarthritis

Osteoarthritis (OA) results from the progressive degeneration of articular cartilage. Although multiple factors have been identified that contribute to the disease onset, the primary risk factor is aging.

In the present study, we have proposed a novel pathway that may explain the mechanism by which age contributes to the progression of OA.

Advanced Glycation End products (AGEs) are products of the glycation reaction between reducing sugars and proteins. AGEs accumulate with age in various tissues including cartilage. They affect biochemical and biomechanical properties of the cartilage and stimulate the release of an inflammatory chemical, TNF-α from cartilage cells.

We believe that TNF-α increases cell-cell communication through proteins known as connexins (Cx), especially Cx43. Cx43 in turn spreads the inflammatory response and inhibits collagen synthesis by cartilage cells. The effects of AGEs are thought to be mediated via its receptors (AGERs).

We are currently studying the role of AGEs, putative AGE receptors and connexin-mediated inflammatory responses using human OA cartilage tissue and a mouse chondrocyte cell line.

People involved:

Dr Shamin Shaikh (PI)
Dr Sue McGlashan (PI)
Mr Benedict Uy

Clinical collaborator:

Mr Anthony Hardy

Funded by:

The University of Auckland Faulty of Medical and Health Sciences Faculty Development Research Fund

rage_fig1_300px

Total AGE in human articular cartilage. Total AGE staining was intense in cells (arrow) and in the ECM (arrowheads).

 

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