References


Biobank lab Jena

Previous Studies - pre-dating but laying the foundations for the establishment of the HGB

*Dragunow M. The adult human brain in preclinical drug development. Nature Reviews Drug Discovery, 7, 659-666, 2008. This manuscript provides the blue-print/theoretical basis for establishing both the Hugh Green Biobank and NeuroValida.

Gibbons H, Dragunow M. Adult human brain cell culture for neuroscience research. The International Journal of Biochemistry and Cell Biology 42, 844-856, 2010.

*Dragunow M. High content analysis in neuroscience. Nature Reviews Neuroscience 9(10), 779-88, 2008. This manuscript describes the high content analysis/screening techniques utilized by scientists studying human brain cell cultures and for the drug screening work of HGB scientists and Neurovalida.

Gibbons H, Hughes S, Van Roon-Mom W, Greenwood J, Narayan P, Teoh H,  Bergin P, Wood P, Mee E, Faull RLM, Dragunow, M. Cellular composition of human glial cultures from adult biopsy brain tissue. Journal of Neuroscience Methods 166, 89-98, 2007. Our first research publication growing human brain cells.

Narayan P, Dragunow M. High Content Analysis of histone acetylation in human cells and tissues. Journal of Neuroscience Methods 193(1): 54-61, 2010.

Gibbons H, Smith A, Teoh H, Bergin P, Mee E, Faull RLM, Dragunow M. Valproic acid induces microglial dysfunction, not apoptosis, in human glial cultures. Neurobiology of Disease 41, 96-103, 2011.

Narayan P, Gibbons H, Mee E, Faull R, Dragunow M. High throughput quantification of cells with complex morphology in mixed cultures. Journal of Neuroscience Methods 164(2): 339-49, 2007

Park T, Monzo H, Mee EW, Bergin PS, Teoh HH, Montgomery JM, Faull RLM, Curtis MA, Dragunow M. Adult Human Brain Neural Progenitor Cells (NPCs) and Fibroblast-Like Cells Have Similar Properties In Vitro but Only NPCs Differentiate Into Neurons. PLoS One, 92(4):841-9, 2012.

HGB supported studies

Smith A, Gibbons H, Oldfield RL, Bergin PM, Mee EW, Faull RLM, Dragunow M. The transcription factor PU.1 is critical for viability and function of human brain microglia. Glia 61(6):929-42, 2013.

Smith A, Gibbons H, Oldfield R, Bergin P, Mee E, Curtis M, Faull RLM, Dragunow M. M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia. Journal of Neuroinflammation 10: 85. doi: 10.1186/1742-2094-10-85, 2013

Smith A, Graham S, Feng S, Oldfield R, Bergin P, Mee E, Faull RLM, Curtis M,  Dragunow M. Adult human glia, pericytes and meningeal fibroblasts respond similarly to IFNg but not to TGFβ1 or M-CSF. PLoS One 2013 Dec 5;8(12):e80463

Jansson D, Rustenhoven J, Feng S, Hurley D, Oldfield RL, Bergin PS, Mee EW, Faull RLM, Dragunow M. A role for human brain pericytes in neuroinflammation. Journal of Neuroinflammation 11:104 DOI: 10.1186/1742-2094-11-104, 2014.

Rustenhoven J, Scotter E, Jansson D, Kho D, Oldfield R, Bergin PS, Mee E, Faull RLM, Curtis M, Graham S, Park T, Dragunow M. An anti-inflammatory role for CEBPδ in human brain pericytes. Scientific Reports 5:12132. doi: 10.1038/srep12132, 2015

Park T, Waldvogel H, Montgomery J, Mee E, Bergin P, Faull RLM, Dragunow M, Curtis M. Chapter 27. Identifying neural progenitor cells in the adult human brain. In Neural Progenitor Cells: Methods and Protocols B Reynolds and L. Deleyrolle (Eds.). Springer Science+Business Media New York, 1059:195-225, 2013.

Smith A, Gibbons H, Lill C, Faull RLM, Dragunow M. Isolation and culture of adult human microglia within mixed glial cultures for functional experimentation and high content analysis. In Microglia: Methods in Molecular Biology Edited by Bertrand Joseph and José Luis Venero, Humana Press, NY, Volume 1041, 2013, pp 41-51.

Dieriks BV, Park TH, Fourie C, Faull RLM, Dragunow M, Curtis M. α-synuclein transfer through tunneling nanotubes occurs in SH-SY5Y cells and primary brain pericytes from Parkinson’s disease patients. Scientific Reports 2017 Feb 23;7: 42984. doi: 10.1038/srep42984.

Dragunow M, Feng S, Rustenhoven J, Curtis M, Faull RLM. Studying human brain inflammation in leptomeningeal and choroid plexus explant cultures. Neurochemical Research 41(3) 579-588, 2016.

Rustenhoven J, Park T, Schweder P, Scotter J, Correia J, Smith A, Gibbons H, Oldfield R, Bergin P, Mee E, Faull RLM, Curtis M, Graham ES, Dragunow M. Isolation of highly enriched primary human microglia for functional studies.  Scientific Reports, 26: 19371. doi: 10.1038/srep19371 2016.

Rustenhoven J, Aalderink M, Scotter E, Oldfield R, Bergin P, Mee E, Graham ES, Faull RLM, Curtis M, Park T, Dragunow M.  TGFβ1 regulates human brain pericyte inflammatory processes involved in neurovascular function. Journal of Neuroinflammation, 6: 26587. doi: 10.1038/srep26587, 2016.

Park T, Feisst V, Brooks A, Rustenhoven J, Monzo H, Feng S, Mee E, Bergin P, Oldfield R, Graham ES, Curtis M, Faull RLM, Dunbar R, Dragunow M. Cultured pericytes from human brain show phenotypic and functional differences with differential CD90 expression. Scientific Reports 6:26587. doi: 10.1038/srep26587, 2016.

Jansson D, Scotter E, Rustenhoven J, Coppieters N, Smyth L, Oldfield RL, Bergin PS, Mee EW, Faull RLM, Dragunow M Interferon blocks signaling through PDGFRb in human brain pericytes. Journal of Neuroinflammation 13: 249, 2016.

Rustenhoven J, Smyth L, Jansson D, Schweder P, Aalderink M,  Scotter E, Mee E, Faull RLM, Park T, Dragunow M. Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction. BMC Neuroscience (in press, 10/2/18).

Smyth L, Rustenhoven J, Park T, Schweder P, Jansson D, Heppner, O'Carroll S, Mee E, Faull RLM, Curtis M, Dragunow M. Unique and shared inflammatory profiles of human brain endothelia and pericytes Journal of Neuroinflammation (in press).

Dragunow M. Meningeal and Choroid Plexus Cells – Novel Drug Targets for CNS Disorders. Brain Research 1501, 32-55, 2013.

Smith A and Dragunow M. The human side of microglia. Trends in Neurosciences 37(3) 125-135, 2014.

Rustenhoven J, Jansson D, Smyth L, Dragunow M. Brain pericytes as mediators of neuroinflammation. Trends in Pharmacological Sciences 38(3):291-304, 2017.

Rustenhoven J, Smith A, Smyth L, Jansson D, Scotter E, Swanson M, Alderink M, Coppieters N, Narayan P, Handley R, Overall C, Park T, Schweder P, Heppner P, Curtis M, Faull RLM, Dragunow M.  PU.1 regulates Alzheimer's disease-associated genes in primary human microglia Molecular Neurodegeneration 13(1): 44, 2018

Conference presentations and invited talks

Dragunow M, Smith A. A high content assay to measure microglial morphology. Australian Neuroscience Society Annual Meeting Gold Coast, Australia, 29 January - 1 February 2012.

Dragunow M, Smith A, Feng S. An in vitro model of human meningeal inflammation. 8th FENS Forum of Neuroscience, Barcelona, July 14-18, 2012.

MacVeigh D, Dragunow M. Inflammation and insulin signalling in the human brain: investigating the link between Diabetes and Alzheimer’s disease. National Research Centre for Growth and Development Science Symposium, Palmerston North, New Zealand, 20-22 November 2012.

Smith A, Dragunow M. The Inducible Expression of HLA in Adult Human Glia. 11th International Congress of Neuroimmunology (ISNI). Boston (MA) USA November 4 to 8, 2012.  

Jansson D, Coppieters N, Dragunow M. Insulin resistance in human neurons. Gordon Research Conference - Oxidative Stress & Disease - The Metabolic-Inflammatory Axis in Brain Aging and Alzheimer's Disease April 14-19, 2013, Les Diablerets Conference Center, Les Diablerets, Switzerland.

Jansson D and Dragunow M. Modelling the effects of systemic inflammation on the central nervous system in primary human brain cells. Gordon Research Conference - Oxidative Stress & Disease - The Metabolic-Inflammatory Axis in Brain Aging and Alzheimer's Disease April 14-19, 2013, Les Diablerets Conference Center, Les Diablerets, Switzerland.

Dragunow M. Invited talk to the Motor Neurone Disease Association of New Zealand Annual General Meeting, Tauranga, Saturday 19 October, 2013 where is presented the Guest Lecture entitled “Neurodegeneration Research – MND”

Rustenhoven J, Park T, Jansson D, Oldfield R, Bergin PS, Mee EW, Faull RLM, Dragunow M. The transcription factor C/EBPδ regulates human brain pericyte inflammatory responses. Society for Neuroscience Washington DC, USA, November 2014.

Jansson D, Rustenhoven J, Oldfiled R, Bergin PS, Mee EW, Faull RLM, Dragunow M. Proinflammatory cytokines alter signalling in human brain pericytes. Society for Neuroscience Washington DC, USA, November 2014.

Scotter E, Rustenhoven J, Dieriks V, Park T, Dragunow M. Dysfunction of pericyte cells of sporadic ALS patients. Cold Spring Harbor Meeting on Blood Brain Barrier, December 10 - 13, 2014.

Aalderink M, Curtis M, Faull RLM, Dragunow M. MicroRNA regulation of human brain pericytes. Alzheimer’s Disease International Conference (AAIC), Washington DC, USA, 18-23 July, 2015.

Correia J, Park T, Rustenhoven J, Monzo H, Dieriks V, Mee E, Faull RLM, Curtis MA, Dragunow M. Characterisation of Glioblastoma Multiforme derived pericytes and evaluation of response to Mitogen activated/extracellular signal regulated kinase (MEK) pathway inhibition. Neurosurgical Society of Australasia Annual Scientific Meeting, Auckland, 30 Sept-2 Oct 2015.

Park T, Correia J, Mee E, Monzo H, Rustenhoven J, Faull RLM, Curtis MA, Dragunow M. MEK inhibitor U0126 inhibits glioma cancer stem cell proliferation and migration. Neurosurgical Society of Australasia Annual Scientific Meeting, Auckland, 30 Sept-2 Oct 2015.

Rustenhoven J, Scotter E, Smyth L, Park T, Curtis M, RLM. Faull, Graham S and Dragunow M. Phagocytosis by human brain pericytes: implications for Alzheimer’s disease. World Preclinical Congress: Blood-Brain Barrier, Boston, MA, USA, 2016.

Rustenhoven J, Scotter E, Smyth L, Park T, Curtis M, Faull RLM, Graham S and Dragunow M. Phagocytosis by human brain pericytes: implications for Alzheimer’s disease. Gordon Research Conference: Barriers of the CNS, New London, NH, USA, 2016.

Jansson D, Scotter E, Rustenhoven J, Coppieters N, Smyth L, Faull RLM, Dragunow M. Interferon-γ blocks signalling through PDGFRβ in human brain pericytes. World Pre-Clinical Congress: Blood-brain barrier. Boston, MA, USA, 2016.

Jansson D, Scotter E, Rustenhoven J, Coppieters N, Smyth L, Faull RLM, Dragunow M. Interferon-γ blocks signalling through PDGFRβ in human brain pericytes. Gordon Research Conference: Barriers of the CNS, New London, NH, USA, 2016.

Park T, Kim E, Smyth L, Rustenhoven J, Faull RLM, Dragunow M. Aberrant pericyte signaling in Huntington disease. AWCBR, Queenstown, New Zealand, August 2016.

Joret M, Park T, Schweder P, Mee E, Kim B, Faull RLM Dragunow M. Pericyte immunosuppression in glioblastoma multiforme. AWCBR, Queenstown, New Zealand, August 2016.

Smyth L,  O’Carroll S, Rustenhoven J, Curtis M, Park T, Dragunow M. Transforming growth factor-β1-stimulated brain pericytes negatively regulate the endothelial cell barrier in vitro. The fifth Cold Spring Harbor conference on Blood Brain Barrier (7 – 10 December, 2016), USA.

Dragunow M. Human brain cell culture from neurosurgical specimens. An invited talk delivered to the Workshop – “Modelling Building and Interrogating Neural Networks – An ACES event” University of Wollongong Australia, October 3 2017.

Aalderink M, Smith A, Rustenhoven J, Dragunow M. Actions of miR-124 on human brain cells. The XIII European Meeting on Glial Cells in Health and Disease, July 8-11 2017, Edinburgh.

Jansson D, Aalderink M, Rustenhoven J, Scotter EL, Dragunow M. Drug screening in primary human brain cells to target neuroinflammation. 12th International Conference on Cerebral Vascular Biology, Monash University, Melbourne, 28 November – 1 December 2017.

Smyth L, Jansson D, Rustenhoven J, Park T, Dragunow M. Platelet-derived growth factor-BB induces a rapid inflammatory response in human brain pericytes. 12th International Conference on Cerebral Vascular Biology, Monash University, Melbourne, 28 November – 1 December 2017.

Rustenhoven J, Jansson D, Feng S, Aalderink M, Dragunow M. Ex vivo Human leptomeningeal explants for neuroinflammatory research. 12th International Conference on Cerebral Vascular Biology, Monash University, Melbourne, 28 November – 1 December 2017.

Ganesh M, Clare A, Dragunow M, Hughes S, Clarkson A, Empson R. Pericyte therapeutics to repair stroke lesioned brain. Brain Health Research Centre Conference, University of Otago, Dunedin New Zealand, 8 June 2017.

Ganesh M, Clare A, Dragunow M, Hughes S, Clarkson A, Empson R. Pericyte therapeutics to repair stroke lesioned brain. AWCBR, Queenstown, New Zealand, September 2017.

Park, T, I-H., Rustenhoven, J., Smyth, L., Lee, K, Y-J., Schweder, P., Jansson, D., Dieriks, V., Jung, Y-W., Montgomery, J., Dragunow, M. Growing primary adult human brain cells to study neurological diseases. Cold Spring Harbor Laboratory Conference on Development and 3-D modelling of the human brain. December 6 - 9, 2017, Cold Spring Harbor, New York, USA.

Jansson D, Smyth L, Rustenhoven J, Scotter EL, Dragunow M. PDGFRß-dependent signalling in human brain pericytes. Barriers of the CNS Gordon Research Conference - Exploring Novel Technologies to Overcome Present Challenges in Understanding Brain Barriers Function, Colby-Sawyer College, New London, NH 03257, United States, June 17 - 22, 2018.

Smyth L, Rustenhoven J, Jansson D, Park T, Dragunow M. Inflammatory responses are differentially regulated in blood-brain barrier pericytes and endothelia. Barriers of the CNS Gordon Research Conference - Exploring Novel Technologies to Overcome Present Challenges in Understanding Brain Barriers Function, Colby-Sawyer College, New London, NH 03257, United States, June 17 - 22, 2018.

Rustenhoven J, Smith A, Smyth L, Jansson D, Copieters N, Narayan P, Park T, Schweder P, Heppner P, Curtis M, Faull RLM, Dragunow M. The transcription factor PU.1 regulates a network of innate and adaptive immune mediators in primary human microglia: implications for Alzheimer’s disease. Keystone Symposia – Advances in Neurodegenerative diseases research and therapy & New Frontiers in Neuroinflammation: what happens when the CNS and periphery meet? June 17-21, 2018 Keystone, Colorado, USA.

Shim, V. B., Li, S. & Dragunow, M. Parametric analysis of the pattern of damage in traumatic brain injuries. 8th world Congress of Biomechanics, Dublin Ireland, (2018).

Siemens AD, Jansson D, Curtis MA, Faull RLM, Dragunow M, Scotter E (2018). Identification of RNA foci in cultured C9ORF72-positive human spinal cord pericytes. Australian Motor Neurone Disease Symposium, Melbourne Convention and Exhibition Centre, Melbourne, 22–24 March 2018.

Park, T, I-H., Lee, K, Y-J., Schweder, P., Dieriks, B. V., Jung, Y-W., Montgomery, J., Dragunow, M. Growing primary adult human neurons to study neurological diseases. Cold Spring Harbor Asia – Latest Advances in Development & Function of Neuronal Circuits, Awaji Yumebutai Conference Center, Japan, September 25-28, 2018.

Thomas I-H. Park, Bernard Kim, Jena Macapagal, Victor Dieriks, Patrick Schweder, Peter Heppner, Clinton Turner, Maurice Curtis and Mike Dragunow. PSA-NCAM in Glioblastoma – A negative prognostic marker and a therapeutic target? 23rd Annual Scientific Meeting of the Society for Neuro-oncology, Marriott Hotel, New Orleans, Louisiana, USA Thursday, November 15 - Sunday, November 18.

Macapagal J, Park T, Joret M.O,Rustenhoven J, Dieriks B.V., Faull R.L.M, Schweder P, Dragunow M. The potential contribution of pericytes to GBM tumour micro-environment immunosuppression via dampened expression of ICAM-1, VCAM-1 and MCP-1. 23rd Annual Scientific Meeting of the Society for Neuro-oncology, Marriott Hotel, New Orleans, Louisiana, USA Thursday, November 15 - Sunday, November 18.