CBR Seminar: Dr Juliette Cheyne Event as iCalendar

25 January 2018

10:30 - 11:30am

Venue: CBR Seminar Room 501-505

Location: Faculty of Medical and Health Sciences

Host: Dr Juliette Cheyne

Contact info: Frankie Favero

Contact email: f.favero@auckland.ac.nz

IMG_0311

Learning from the experts: advances in in vivo recording in the CBR, with help from Janelia

During this seminar I will give an overview of the in vivo recording techniques that are currently established in the CBR and give an update on the planned future advances including miniscope and two-photon recordings. In vivo wide-field calcium imaging and whole cell electrophysiology are currently established in the CBR.

These techniques allow neuronal activity to be probed in the intact system which is important to bridge the translational gap from molecular mechanisms to functional consequences, and clinical outcomes. These technologies are useful for answering a number of questions about how the brain functions at the network and synaptic level.

In November 2016 I visited Janelia, on the outskirts of Washington DC to learn more about ways to further develop these techniques. The Howard Hughes Medical Institute founded Janelia in 2006 with the idea of providing an alternative innovative research environment to drive science forward. Janelia currently has an emphasis on two research areas: developing new imaging methods and discovering the basic principles underlying computation by neuronal circuits.

Today, Janelia includes 50 labs, nine project teams, and additional scientific support staff. Many of these labs specialise in in vivo recordings (e.g. Svoboda, Magee, Sprusten, Lee). Visiting Janelia gave me insights into the best ways to achieve chronic in vivo recordings.

Together with Johanna Montgomery's lab we are working towards establishing miniscope recordings, which allow activity to be imaged chronically in the brain.  In addition, through a collaboration with the Physics Department we are working towards building a customized two-photon imaging system which will allow activity to be recorded at the cellular and even synaptic level.  Together with the ongoing development of optogenetic techniques within the CBR this would give researchers the possibility to control and record activity inside the brain.

These technologies could be applied to many different disease models that are currently used in within the CBR including Parkinson’s disease, Alzheimer’s disease and Huntington’s disease. 

 

About Dr Juliette Cheyne

Juliette did her BSc (Biomedical Science), Honours and PhD at the University of Auckland. She then I moved to Amsterdam to pursue a Postdoctoral Fellowship at the Netherlands Institute for Neuroscience, where she learned in vivo recording and imaging techniques.

In 2016 she moved back to Auckland to work with her PhD Supervisor Associate Professor Johanna Montgomery and also Professor Peter Thorne. Together, they aim to better understand the development and function of the auditory and prefrontal cortex in in-vivo models of Autism Spectrum Disorder.