Department of Physiology seminar: “Far field fluorescence microscopy at nanometer resolution” Event as iCalendar

28 April 2014

12:30 - 1:30pm

Venue: Seminar Room 503-024, Building 503, 85 Park Road, Grafton

Contact info: For more information and RSVP please contact Megan Spiers on +64 9 923 6720. RSVP by 17 April 2014

Contact email: m.spiers@auckland.ac.nz

Novel developments in optical technology and photophysics made it possible to radically overcome the diffraction limit (ca. 200 nm laterally, 600 nm along the optical axis) of conventional far-field fluorescence microscopy. Presently, three principal “nanoscopy” families have been established: “Nanoscopy” based on highly focused laser beams, like 4Pi-, STED-, and RESOLFT-microscopy; nanoscopy based on Structured Illumination Excitation (SIE), like SMI and SIM; and nanoscopy based on Spectrally Assigned Localization Microscopy (SALM), like PALM/FPALM, SPDM, STORM, dSTORM/GSDIM etc., allowing superresolution down to single molecule resolution even in the case of homogeneous excitation.

With such techniques, it has become possible to analyze the spatial distribution of fluorescent molecules on surfaces and in biostructures with a greatly increased light optical resolution down to a few tens of nanometer in 3D, and a few nanometer in the object plane, corresponding to 1/100 of the exciting wavelength.

Using a novel direct DNA imaging SPDM technique, it became possible to localize up to several million individual DNA molecules in optical sections of mammalian cell nuclei. Application examples obtained by focused, structured, and localization techniques (SPDM) will cover a variety of biostructures, such as membrane complexes, cellular protein distribution, nuclear nanostructures, and “nanoimaging” of individual viruses, including a discussion of perspectives for epigenetics , cancer research, and virology.

 

Presented by: Professor Christoph Cremer

Since 2004 Professor Cremer has been a Director of Nuclear Genome Biophysics, Institute of Pharmacy and Molecular Biotechnology (IPMB), Heidelberg University. 2006-2009 Second Speaker of the Senate of the University. Scientific Member of various institutions in Germany and USA. Since August 2011, head of the Superresolution Microscopy group at the Institute of Molecular Biology (IMB), Mainz; since September 2013, also Honorary Professor (Physics) at the University of Mainz (JGU).

Since the 1970s, development of light-optical microscopy methods for biophysical applications, especially for the analysis of nuclear genome structures.