Molecular Medicine & Pathology seminar: The nuts and bolts of processing and cellular analysis of primary tissue specimens Event as iCalendar

25 November 2013

3:30 - 4:30pm

Venue: Seminar Room 501-505, Building 501, 85 Park Road, Grafton

Compared to other cell biology techniques, flow cytometry is one of the most flexible single cell technologies, applicable to almost any biological problem as long as cells can be prepared in single cell suspensions.  Combining powerful single-cell cytomic analysis of cell’s phenotype and sorting with single cell genomic and transcriptomic profiles and xenografting, enables us to understand tissue regeneration, tumor evolution and to determine which factors drive tumor aggressiveness.  This presentation discusses the cell analysis needs of research and clinical cellular therapy laboratories, particularly with respect to the challenges facing the development or adaptation of home-brew cytometric assays of purity and identity.   The discussion will be accompanied by practical suggestions for disaggregation of tissues into single cell suspensions.  We will focus on avoiding some of the technical pitfalls encountered when performing multi-dimensional flow cytometry on disaggregated tissues. These include recognizing sources of bias in cell recovery, use of doublet discrimination, use of DAPI to detect and removing hypodiploid events, coping with autofluorescence, and optimal use of dump gates and analytical strategies for multivariate data where number of samples is smaller than the number of measured parameters.

Presented by: Associate Professor Vera S. Donnenberg & Professor Albert D. Donnenberg

Professor Albert Donnenberg is a Professor of Infectious Disease and Microbiology in the Graduate School of Public Health and a Professor of Medicine in the School of Medicine at the University of Pittsburgh. He is also an Adjunct Professor of Biotechnology at the Université Paris Diderot. In addition to teaching, Dr. Donnenberg is the Director of the University of Pittsburgh Cancer Institute Flow Cytometry Facility as well as both the UPMC Hematopoietic Stem Cell Laboratory and the University of Pittsburgh Cancer Institute Flow Cytometry Facility. He is also the Co-Leader of the UPCI Cancer Stem Cell Program.

Dr. Donnenberg's research focuses are: immunologic consequences of autologous transplantation in systemic sclerosis; haplo-identical HSCT; use of bone marrow derived and peripheral blood derived stem and progenitor cells for regenerative therapy; the cancer stem-cell hypothesis; the identification of therapeutic targets on cancer stem cells; and technological advances in flow cytometry. He is on the editorial board of Clinical and Applied Immunology Reviews, and he is certified as an inspection team leader of the College of American Pathologists.

Associate Professor Vera Donnenberg is an Associate Professor of Cardiothoracic Surgery in the School of Medicine at the University of Pittburgh. Her research focuses on flow cytometry, broncho-alveolar lavage fluid, and the analysis of dendritic cell subsets. Dr. Donnenberg has written over 75 publications, abstracts and other scientific presentations.

Dr. Donnenberg is a member of several professional and scientific societies including the American College of Clinical Pharmacology, American Association for Cancer Research, and the International Society for Analytical Cytology

Anyone wanting to meet with Drs Vera or Albert Donnenberg should email Stephen Edgar

For more information please contact: Robyn McDonald Email: Phone: 373 7599 ext 86285