Biomedical Imaging Research Unit seminar: Non invasive 3D and 4D characterization of buried and hierarchical structures in soft & hard materials with a lab based X-Ray Microscope (XRM) Event as iCalendar

18 February 2013

2 - 3pm

Venue: Seminar Room 503-020, Building 503, 85 Park Road, Grafton

While electron microscopy and fluorescence confocal microscopy had provided a lot of insight in our understanding of biological systems, there is an ongoing gap in integrating and interpreting the results from these molecular resolution 2D imaging data to the larger scale hierarchical structure and functions of the tissue and cells within a complex 3D organism. Moreover, most imaging modalities require destructive sample preparation steps, which often involve sectioning and staining. As these imaging modalities are in 2D, it is also diffi cult to characterize accurately buried 3D microstructures, porosity, size, textual distribution and defects within samples. We describe a suite of lab based X-ray Microscope (XRM) with similar architecture to synchrotron based systems, which are capable of rapid multiscale morphological characterization from whole organism, tissue (plant and animal), cell to organelle level in 3D. Soft material characterization is diffi cult with almost all imaging modalities - from optical microscopy to SEM or TEM, which often require time consuming and extensive sample preparation. By employing phase contrast techniques similar to its synchrotron counterpart, imaging contrast in XRM for soft materials such as polymers in 3D can be increased substantially and at high resolution. For many specimen types, this can be done with little to no sample preparation and with or without staining protocols.

Furthermore 3D results of surface and buried structures are obtained at a fraction of time compared to histology or electron tomography. Data from the lab based XRM will be presented and compared to those obtained from synchrotron XRM, Histology, SEM, TEM and visible microscopy. Hierarchical multiscale imaging in genomics, developmental biology, agriculture, and material science will be illustrated with imaging of fruits and food (AS IS), whole zebra fi sh, whole embryo mounts, mapping of neurons network in the brain, mapping of vasculature and 3D morphology in tissue and organs in knock outs, fuel cells and desalination membranes. Other examples include modeling of fl ow in biomaterials for drug delivery, soil, rocks and engineered materials. The novel use of XRM as a navigation guide in preparation of FIB-SEM or TEM lamella specimen after molecular imaging and gene tagging in confocal microscopy will also be shown. A discussion on correlative microscopy and the emerging fi eld of in situ 4D imaging in biomechanical and thermal mechanical studies will also be given.