Laser line |
Detector |
Mirror |
Emision filter |
Fluorophores |
355nm |
Trigon |
|
450/50 BP |
Indo-1 (Violet), Alexa Fluor 350, DAPI, Hoechst, DyeCycle Violet,
Pacific Blue |
355nm |
Trigon |
505 LP |
530/30 BP |
Indo-1 (Blue), Pacific Orange |
|
|
|
|
|
488nm |
Octagon |
505 LP |
530/30 BP |
Alexa Fluor 488, FITC, GFP, Qdot 525, BODIPY, CFSE, CMFDA,
DyeCycle Green, Eosin, FcOxyBURST Green, Fluo-3, Fluo-4, Live/Dead Fixable Green, SYBR Green, SYTOX Green, ZsGreen1 |
488nm |
Octagon |
550 LP |
575/26 BP |
DsRed, PE, Qdot 565, Qdot 585, 575/26, Cy3, dTomato, DyeCycle Orange, PI |
488nm |
Octagon |
600 LP |
610/20 BP |
PE-Texas Red, Qdot 605, Nile Red, PE-Alexa Fluor 610, PI |
488nm |
Octagon |
685 LP |
695/40 BP |
PE-Cy5, PerCP, PerCP-Cy5-5, PI, 7-AAD, PE-Alexa Fluor 700, PE-Cy5-5,
Qdot 705 |
488nm |
Octagon |
735 LP |
780/60 BP |
PE-Cy7, PE-Alexa Fluor 750, Qdot 800 |
|
|
|
|
|
633nm |
Trigon |
|
660/20 BP |
APC, Alexa Fluor 633, Alexa Fluor 647, Alexa Fluor 660,
Live/Dead Fixable Far Red, SYTOX Red, TO-PRO 3 |
633nm |
Trigon |
735 LP |
780/60 BP |
Live/Dead Fixable Near-IR, APC-Cy7, APC-H7, Alexa Fluor 750,
APC-Alexa Fluor 750, DRAQ5 |