High throughput and high content screening machine
Discovery-1 is an automated
inverted fluorescence and brightfield microscope and image analysis system, which
uses the MetaMorph software package.
revolutionising the study of
the complex “orchestra” of signals that permeate between and within cells (Signal
transduction research). This type of analysis is called High Content Analysis (HCA)
which is best described as the combined use of high throughput (HTP) microscopy
(Discovery-1) and HTP image analysis (MetaMorph) to quantify cellular information
such as morphology, signal intensity and signal location in a cell or organelle
within a cell. Discovery-1 and MetaMorph can measure both the level of a signalling
molecule and its anatomical location within a cell very quickly, accurately and
objectively. It has many important
applications in signal transduction research such as:
- Studying how cell membrane-bound receptors for hormones, neurotransmitters
and drugs work by measuring at HTP, the movement of receptors from cell membranes
- Measuring at HTP, the translocation of a transcription factor (eg:
NFkB) from cytosol to nucleus.
- Tracing and quantifying at HTP, the complex processes
(axons and dendrites) of nerve cells.
- Counting cells or organelles within cells
- Measuring cell death and cell division at HTP.
A number of in-house
assays have also been developed including:
- Counting and measuring the morphology
of various types of cells.
- Quantifying the migration of cells.
- Measuring fat deposits
in cells and tissues.
- Measuring abnormally aggregated proteins.
server has been set up in the Department of Pharmacology to
store and archive the terabytes of information generated by Discovery-1.
Discovery-1 and HCA analysis can be used in drug screening and chemical, genetic
and functional genomic studies and is thus a powerful tool in biomedical and biological
Discovery 1 assays
- Fast and automatic acquisition of images from tissue culture plates
- Efficient analysis of images
- Several assays available to study cell properties
- Ability to visualise and quantify biological entities unseen by the naked eye
For more information, please see the Contacts page.
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