Cell detection
Counts cells/specific cell compartments based on size and staining intensity.
C6 cell nuclei stained with Hoechst, acquired using Dapi UV filter.
Translocation
Quantifies the translocation of a specific molecule from one compartment of a cell
to another compartment. For example, the movement of NFκB from the cytoplasm to
the nucleus of BV2 cells upon exposure to LPS.
Before LPS treatment
After LPS treatment (hold mouse image over for description)
Neurite outgrowth
Quantifies the average number or length of processes sprouting from neurons as well
as their branching (complexity). This assay can be used to determine specific drug
effects on cultured neurons.
Gap area
This image stack shows 60 wells from a 96 well plate. The confluent C6 cells in
each well were scratched (physical insult) and grown in different conditions. Using
Metamorph, the area of the gap (orange) remaining after the drug treatments can
be quantified for each well and used to determine different drug effects on the
rate of cell migration.
This assay can be used to quantify cell migration in 2-dimensional scratch motility
assays. Useful as an initial screen for anti-tumour agents.
Count Nuclei for Hoechst/Dapi stained cells
Useful for counting total cells.
Angiogenesis
This assay looks at tube formation and allows analysis of vessel growth.
Live dead
This cell assay is an ideal method to quantify drug effects on cell viability.
Cell health
Can sort cells into healthy, necrotic and apoptotic categories.
Mitotic index
A quick and accurate analysis of cellular proliferation at high throughput.
Cell scoring
Allows 2 wavelengths to be processed simultaneously and has many applications. For
example, we have used this assay successfully to count numbers of protein aggregates
in cells at high through-put.
Granularity
Useful for receptor internalization studies.
GPCR cycling
High content analysis of receptor internalization.
Tissue micro array
Tissue micro array acquisition and analysis is also available.
Tailor made assays
High throughput tissue morphometrics
In addition to providing a suite of High Content Analysis (HCA) assays for analysing
signal transduction and cell morphometrics in cell culture formats, we also have
a tissue analysis facility.
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