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Laboratory Directors

Prof Kathryn Crosier Professor, Clinical Molecular Medicine & Pathology School of Medical Sciences Phone: +64 9 373 7599 ext 86043; 9236043 Email: ke.crosier@auckland.ac.nz

Prof Phil Crosier Professor Molecular Medicine & Pathology School of Medical Sciences Phone: +64 9 373 7599 ext 86279 Email: ps.crosier@auckland.ac.nz

       

Graduate Students	Staff
Michael Haggerty Kazuhide Okuda Leslie Sanderson	Chris Hall (Senior Research Fellow) Jonathan Astin (Research Fellow) Alice Domenichini (Research Fellow) Alhad Mahagaonkar (Zebrafish Facility Manager) Annie Chien (Senior Research Technician) Alisha Malik (Senior Research Technician) Pauline Misa (Senior Research Technician) Sophie Wicker (Senior Research Technician) Shantanu Patke (Research Technician) Graham Stevens (Oncologist)

Funding

• Auckland Medical Research Foundation
• Foundation for Research Science and Technology
• Genesis Oncology Trust
• Health Research Council of New Zealand
   

Research

Our laboratory works in the areas of hematopoiesis, vasculogenesis and immunity. We have a particular interest in the Runx family of transcription factors and are investigating their roles in developmental processes and disease. The zebrafish is used by our group as a model system, offering powerful genetic and embryological experimental approaches in a vertebrate. The system has become very popular internationally for biomedical research, with many discoveries providing important insights into human disease. A genetic screen looking for modifiers of Runx1 expression has been undertaken and we are continuing to work on a model of RUNX1-ETO leukemia developed in our laboratory. Investigations are also directed at understanding the gut immune system and opening up opportunities to further understanding of inflammatory bowel disorders.

 

Relevant publications

1. Flores, M.V., Crawford, K.C., Pullin, L.M., Hall, C.J., Crosier, K.E. and Crosier, P.S. (2010) Dual oxidase in the intestinal epithelium of zebrafish larvae has anti-bacterial properties.  Biochem Biophys Res Commun 400, 164-168.
2. Rhodes, J.M., Bentley, F.K., Print, C.G., Dorsett, D., Misulovin, Z., Dickinson, E.J., Crosier, K.E., Crosier, P.S. and  Horsfield, J.A. (2010) Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved. Dev Biol 244, 637-649.
3. Flores, M.V., Hall, C.J., Crosier, K.E. and Crosier, P.S. (2010) Visualization of embryonic lymphangiogenesis advances the use of the zebrafish model for research in cancer and lymphatic pathologies. Dev Dyn 239, 2128-2135.
4. Lam, E.Y., Hall, C.J., Crosier, P.S., Crosier, K.E. and Flores, M.V. (2010)  Live imaging of Runx1 expression in the dorsal aorta tracks the emergence of blood progenitors from endothelial cells.  Blood 116, 909-914.
5. Oehlers, S.H., Flores, M.V., Hall, C.J., O'Toole, R., Swift, S., Crosier, K.E. and Crosier, P.S. (2010) Expression of zebrafish cxcl8 (interleukin-8) and its receptors during development and response to immune stimulation. Dev Comp Immunol 34, 352-359.
6. Hall, C. and Crosier P. (2010) Editorial: Maintaining the balance - fishing for drugs to treat persistent neutrophilic inflammation. J Leukoc Biol 87, 189-191.
7. Brannon, M.K., Davis, J.M., Mathias, J.R., Hall, C.J., Emerson, J.C., Crosier, P.S,, Huttenlocher, A., Ramakrishnan and Moskowitz, S.M. (2009) Psuedomonas aeruginosa type III secretion system interacts with phagocytes to modulate systemic infection of zebrafish embryos.  Cell Microbiol 11, 755-768.
8. Lam, E.Y., Chau, J.Y., Kalev-Zylinska, M.L., Fountaine, T.M., Mead, R.S., Hall, C.J., Crosier, P.S., Crosier, K.E. and Flores, M.V. (2009) Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors.  Blood 113. 1241-1249.
9. Hall, C., Flores, M.V., Crosier, K. and Crosier, P. (2009) Live imaging of zebrafish leukocytes.  Methods Mol Biol 546, 255-271.
10. Stoletov, K., Fang, L., Choi, S.H., Hartvigsen, K., Hansen, L.F., Hall, C., Pattison, J., Juliano, J., Miller, E.R., Almazan, F., Crosier, P., Witztum, J.L., Klemke, R.L. and Miller, Y.I. (2009) Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish. Circ Res 104, 952-960.
11. Hall C., Flores, M.V., Chien, A., Davidson, A., Croisier K. and Crosier P. (2009) Transgenic zebrafish reporter lines reveal conserved Toll-like receptor signaling potential in embryonic myeloid leukocytes and adult immune cell lineages. J Leukoc Biol 85, 751-765.
12. Liongue, C., Hall, C., O'Connell, B.A., Crosier, P. and Ward, A.C. (2009) Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration. Blood 113, 2535-2546.
13. Flores, M.V., Hall, C.J., Davidson, S.J., Singh, P.P, Mahagaonkar, A.A., Zon, L.I., Crosier, K.E. and Crosier P.S. (2008) Intestinal differentiation in zebrafish requires Cdx1b, a functional equivalent of mammalian Cdx2. Gastroenterology 135, 1665-1675.
14. Flores, M.V., Lam, E.Y., Crosier, K.E. and Crosier, P.S. (2008) Osteogenic transcription factor Runx2 is a maternal determinant of dorsoventral patterning in zebrafish. Nat Cell Biol 10, 346-352.
15. Cvejic, A., Hall, C., Bak-Maier, M., Flores, M.V., Crosier, P., Redd, M.J. and Martin, P. (2008) Analysis of WASp function during the wound inflammatory process - live-imaging studies in zebrafish larvae. J Cell Sci 121, 3196-3206.
16. Flores, M.V.C., Hall, C.J., Jury, A., Crosier, K.E. and Crosier, P.S. (2007) The zebrafish retinoid-related orphan receptor (ror) gene family.  Gene Expr Patterns 5, 535-543.
17. Hall, C.J., Flores, M.V.C, Crosier, K.E. and Crosier, P.S. (2007) The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish.  BMC Dev Biol 4, 42.
18. Horsfield, J.A., Anangnostou, S., Hu, K.H., Cho, H.Y., Geisler, R., Lieschke, G.J., Crosier, K.E. and Crosier, P.S. (2007) Cohesin-dependent regulation of runx genes.  Development 14, 2639-2649.
19. Dunn, P.P., Rantes, C., Hall, C., Dyer, C., Compton, J., Hammond, L., Crosier, P. (2007) Identification of a variant HLA-A*3401 allele, A*340102, in an educational DNA typing sample.  Tissue Antigens 70, 518-519.
20. Hall, C.J., Flores, M.V.C., Murrison, G., Crosier, K.E., and Crosier, P.S. (2006) An essential role for zebrafish Fgfrlla during gill cartilage development. Mech Develop 123, 925-940.
21. Flores, M.V.C., Lam, E.Y.N., Crosier, P.S., and Crosier, K.E. (2006) A hierarchy of Runx transcription factors modulate the onset of chondrogenesis in craniofacial endochondral bones in zebrafish. Dev Dyn 235, 3166-3176.
22. Flores, M.V.C., Tsang, V.W.K., Hu, W., Kalev-Zylinska, M.L., Postlethwait, J.H., Crosier, P.S., Crosier, K.E. and Fisher, S. (2004) Duplicate zebrafish runx2 orthologues are expressed in developing skeletal elements. Gene Expr Patterns 4, 573-581.
23. Cattin P. and Crosier P.S. (2004). A nursery that improves zebrafish fry survival. Methods Cell Biol 77, 593-598.
24. Kalev-Zylinska, M.L., Horsfield, J.A., Flores, M.V.C, Postlethwait, J.H., Chau, J.Y.M., Cattin, P.M., Vitas, M.R., Crosier, P.S. and Crosier, K.E. (2003) Runx3 is required for hematopoietic development in zebrafish. Dev Dyn 228, 323-336.
25. Hall, C.J., Flores, M.V., Davidson, A., Crosier, K.E.and Crosier, P.S. (2002) Radar is required for the establishment of vascular integrity in the zebrafish. Dev Biol 251, 105-117. (Work featured on cover)
26. Horsfield, J.A., Ramachandran, A. , Reuter, K. , LaVallie, E. , Collins-Racie, KO , Crosier K.E., and Crosier, P.S. (2002) Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development. Mech Develop 15, 15-26. (Work featured on cover)
27. Crosier P.S., Kalev M.L., Hall C.J., Flores M.V.C., Horsfield J.A. and Crosier K.E. (2002). Pathways in blood and vessel development revealed through zebrafish genetics. IntJ Dev Biol 46, 493-502.
28. Kalev-Zylinska M.L.., Horsfield J.A., Flores M.V.C., Postlethwait J.H., Vitas M.R., Baas A.M., Crosier P.S., and Crosier K.E. (2002) Runx1 is required for zebrafish blood and vessel development, and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis. Development 129, 2015-2030.
29. Crosier P.S., Bardsley A., Horsfield J.A., Krassowska A.K., Lavellie E.R., Collins-Racie L.A., Postlethwait J.H, Yan Y.L, McCoy J.A., and Crosier K.E. (2001). An in situ hybridization screen in zebrafish for the selection of genes encoding secreted proteins. Dev Dyn 222, 637-644.
30. Davidson A.J., Postlethwait J.H., Yan Y.L., Beier D.R., vanDoren C., Foernzler D., Celeste A.J., Crosier K.E., and Crosier P.S. (1999). Isolation of zebrafish gdf7 and comparative genetic mapping of genes belonging to the growth/differentiation factor 5,6,7 subgroup of the TGF-b superfamily. Genome Research 9, 121-129.