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School of Medical Sciences BIRU image competition 2011 - highly commended entries

Confocal laser scanning microscopy

Dr David Crossman

Department of Physiology

Confocal image of rat cardiac arteriole labeled with WGA (green) and phalloidin (red)

Acquired on the Zeiss LSM 710 confocal microscope

Click on thumbnail image to see larger version.

See David receiving his award!

 

 

Light microscopy

Dr Michelle Hunt

Department of Pharmacology & Clinical Pharmacology

C17.2 cells grown in culture that have been fixed with 4% PFA and stained with blue food colouring

Equipment: 20x objective on a Nikon Eclipse TE2000-S microscope .

Click on thumbnail image to see larger version.

See Michelle receiving her award!

 

Confocal laser scanning microscopy

Dr John Taylor & Dr Kristy Manning

School of Biological Sciences

The image is a composite of 3 separate cypovirus polyhedra-GFP  microcrystals incubated with lysotracker dye and imaged by confocal microscopy

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Electron microscopy

LabPlus EM Unit

SAFETY PINS

Equipment BIRU Tecnai G2 Spirit TWIN electron microscope.

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Visualisation and analysis

Emma Kay

Department of Physiology

Live imaging of HEK293 cells expressing human melanocortin 4 receptor tagged with eGFP on Olympus FV1000 LCI microscope. Confocal images were overlaid with DIC images to observe rapid movement of vesicles containing hMC4R-eGFP

Imaged on the Olympus FV1000 Live Cell Imaging Confocal Microscope.

Click on thumbnail image to see larger version.

icon Download Emma's movie (10MB MPEG)

See Emma receiving her award!

 

Light microscopy

Dr Olivia Holland

Department of Obstetrics & Gynaecology

Placental Villus (or the Dying Caterpillar)

Three dimensional reconstruction of a single villus from a human placenta, with the cytoplasm stained green and nuclei stained red. Clusters of nuclei can be seen forming projections from the villus surface. These projections may be newly growing villi or represent the packaging of old nuclei. Click on thumbnail image to see larger version.

See Olivia receiving her prize!

 

Confocal microscopy

Kazuhide Shaun Okuda

Department of Molecular Medicine & Pathology

Lateral image of Tg(lyve1:DsRed2;kdrl:EGFP) transgenic zebrafish line showing the blood vessels (green), lyve1 positive veins (yellow) and lymphatic vessels (red) at 12 days post fertilisation.  The whole larva was imaged Equipment: Nikon D-Eclipse C1 confocal microscope (MMP)

Image in submission for publication - will be uploaded once permission is granted!

See Kazu receiving his award!

 

Electron microscopy

Manpreet Dhami

MICROSCOPIC BIRDS NEST FUNGI

Fruiting bodies of unidentified sooty mould fungi (from Kanuka infested with honeydew scale insect) with characteristic resemblance to ‘Bird’s nest fungi’

Equipment: ESEM FEI Quanta 200F with a SiLi (Lithium drifted) EDS detector Mag: 3000 X

Click on thumbnail image to see larger version.

 



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