Confocal
laser scanning microscopy
Dr
Clairton de Souza
Department of Ophthalmology
Vertical section of Human retina
Acquired on the Olympus FV1000 confocal microscope in the Department of
Ophthalmology
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Light microscopy
Sam Robinson
School of Biological Sciences
Rat hippocampal neuron grown in culture for 2 days. Blue is a DAPI stain of DNA
in the cell showing the cell nucleus, while red is an immunocytochemical stain
for acetylated tubulin (a stable form of the protein) and green is for
tyrosinated tubulin (unstable form of the protein).
Imaged using a Leica DMR microscope with a SPOT pursuit-XS camera in the School
of Biological Sciences.
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Chi-Ying Chou
Department of Ophthalmology
Fluorescein dye in a human eye showing epithelial recovery after a chemical burn
and treatment with connexin43 specific antisense oligonucleotides. The dye
(green) adheres to connective tissue where the epithelium has not yet recovered.
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Electron
microscopy
Linda Graham
LabPLUS
Mast cells
Imaged on the BIRU Tecnai G2 Spirit TWIN electron microscope.
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Visualisation
and analysis
Dr Bridget
Leonard
Department of Physiology & Auckland Bioengineering Institute
3D reconstruction of a section of the left ventricle of rat heart 35 days after
a myocardial infarct.
Imaged on the Large Volume Imaging System in the Department of Physiology.
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Dr Jinny Yoon
Department of Ophthalmology
“Tidal wave” This extensive structure was formed directly in vitro from cells
isolated from the cornea. This structure is reminiscent of the limbus of the eye
where the clear cornea and the white sclera meet. This tidal wave-like structure
is morphologically distinct and retains the labelling of neural precursors which
has been demonstrated in limbal stem cells. Labelled for Musashi-1 (Alexa568,
red), nestin (Alexa488, green) and nuclear counterstain with DAPI (blue).
Image data acquired on the Olympus Fluoview 1000 confocal microscope in the Department of Ophthalmology.
Volume rendering and movie was generated using the AMIRA software.
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