Visualisation and
analysis winner and trophy winner
Chez Viall
Department of Obstetrics and Gynaecology
School of Medicine
This image shows first trimester placental villi that have been cultured for 24
hours. The cytoplasm of cells making up the villi are stained green with Cell Tracker
CMFDA, and nuclei counter-stained with propidium iodide (here white). The z-stack
of the villi was imaged at 20X magnification on the Zeiss LSM 710 inverted confocal microscope (BIRU) and the 3D rendering processed
with Zen software.
Click on the thumbnail image to see a larger version.
See Chez with her prize.
View another image that received a highly commended award
Light microscopy winner
Juliette Cheyne
Department of Physiology,
School of Medical Sciences
Fluorescence image of dissociated hippocampal cultures (21 days in vitro). Dendrites
of neurons stained with microtubule associated protein 2 (MAP2, blue), Golgi stained
with GM130 (red) and the granule cell marker calbindin (green).
Imaged at 63x magnification on the Zeiss Axioplan2
widefield fluorescence microscope
Click on the thumbnail image to see a larger version.
See Juliette with her
prize.
View another fluorescence image that received a highly
commended award
Electron microscopy winner
Vijayaraghavan
Rajagopal
Auckland Bioengineering Institute
A longitudinal view inside a heart cell (acquired with a transmission electron microscope)
using a new tissue preparation method that removes the grid-staining procedure.
Involves HPF-FS.
Preparation and imaging work done in collaboration with Ying Jones at NCMIR (UCSD).
This image shows that you don’t need grid-staining anymore.
Click on the thumbnail image to see a larger version.
See Vijay
receiving his prize.
View another electron microscope image that received a
highly commended award
Confocal
laser scanning microscopy winner
Cameron Johnson,
Department of Opthalmology, School of Medicine
Section of Wistar rat retina following a partial optic nerve transection. Connexin43 (Alexa488; green) and glial fibrillary acidic protein (Cy3; red) label appears highly
concentrated in the nerve fibre and ganglion cell layers. Cell nuclei are labelled
with DAPI (blue). Images were collected at 60x magnification on the Ophthalmology
Olympus FV1000 confocal
microscope.
Unfortunately, Cameron was not available to collect his prize in person.
View another confocal image that received a highly
commended
award
